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Parastagonospora nodorum is a pathogen of wheat that affects yields globally. Previous transcriptional analysis identified a partially reducing polyketide synthase (PR-PKS) gene, SNOG_00477 ( SN477 ), in P. nodorum that is highly upregulated during infection of wheat leaves. Disruption of the corresponding SN477 gene resulted in the loss of production of two compounds, which we identified as ...

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the plant material was ground in the solvent using a vibrating-ball micromill (based on the Retsch MM300 ball mill, modified for microscale applications and purchased from Qiagen, Hilden, Germany). The vibrating ball in the tube was made of stainless steel and had …

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